productdatasheet
Hsp70genes,membersofamultigenefamily,encodeheat-inducIBLe70kDaheat-shockproteinsthatfunctionasmolecularchaperones.Theyhavebeenidentifiedinmostorganellesofeukaryoticcellsaswellasinbacteria.TheN-terminusofHsp70bindsATPwithhighaffinityandtheC-terminusbindsproteinsandpolypeptides.Hsp70sbindnascentpolypeptidesaswellaspartiallyfoldedintermediatesofproteins,thuspreventingtheiraggregationand/ormisfolding.BindingofATPtriggersaconformationalchangethatresultsinthereleaseofboundprotein.Hsp70sfunctioninproteinsynthesis,proteinfoldingandoligomerization,andproteintransport.
ThisantibodyrecognizesinducibleandconstitutiveHsp70/Hsc70ofhuman,mouse,rat,rabbit,monkey,guineapig,hamster,chicken,bovine,canine,ovine,porcine,fish,Xenopus,Drosophila,C.elegans,andplants.Theepitopeisintheregionofaminoacids503-640ofhumanHsp70.
Immunoblotting:useat1ug/ml.Abandof72-73kDaisdetected.
Immunohistochemistry:useat1-10ug/ml.
Immunoprecipitation:1-10ug/ml.
Flowcytometry:useat10ug/ml.
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:HeLacelllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat4oC.Precipitationmayoccuruponfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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