ORDERINGINFORMATION

CatalogNo.:11099

Format:100ugProteinG-purifiedantibodyinPBS,pH7.4,0.1%sodiumazide.

BACKGROUND

MembersoftheHsp70familyaremolecularchaperonesthatfunctioninproteinfolding,transport,maturationanddegradationbybindingtonascentpolypeptidechainsaswellaspartiallyfoldedproteinintermediatesandpreventingtheiraggregationandmisfolding.The70kDaheatshockcognateprotein,Hsc70,iscloselyrelatedbiochemicallyandBIOLOGicallytoHsp70.

SPECIFICATIONSUMMARY

Antigen:ChickenHsp70/Hsp90complex.

Accessionno.:NP_001006686.1

HostSpecies:MouseCloneno.BB70AntibodyClass:IgG2a

Specificity:Thisantibodydetects72and73kDaproteinscorrespondingtothepredictedmolecularmassesofHsp70andHsc70,respectively,onimmunoblotsofhuman,mouse,rat,rabbit,monkey,hamster,guineapig,dog,bovine,sheep,pig,chickenyeast,fish,andXenopussamples.ThisantibodyrecognizestheinducIBLeandconstitutiveformsofHsp70anddoesnotcross-reactwithHsp90.

APPLICATIONS

Immunoblotting:useat

1ug/ml.

Immunohistochemistry:

useat1-5ug/ml.

DetectionofHsp70inHeLacelllysate.

DetectionofHsp70informalin-fixedhumancoloncarcinoma.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DetectionofHsp70informalin-fixedinflamedmousecolon.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.