CatalogNo.:11138(clone5G1-95)Size:100uginPBS(1mg/ml),pH7.4,50%glycerol,0.09%sodiumazide.

PurifiedbyProteinGaffinitychromatography.

Hsp70genesencodeheat-inducIBLe70kDaheatshockproteins.Hsp70bindsATPwithhighaffinityandpossessesweakATPaseactivitywhichcanbestimulatedbybindingtounfoldedproteinsandsyntheticpeptides.ThestructureoftheATPbindingdomaindisplaysmultiplefeaturesofnucleotidebindingproteins.ThebindingofATPtriggersacriticalconformationalchangewhichleadstothereleaseoftheboundsubstrateprotein.TheuniversalABIlityofHsp70stoundergocyclesofbindingtoandreleasefrompartiallyunfoldedproteinsisthebasisoftheirroleinproteinsynthesis,proteinfolding,oligomerization,andproteintransport.

Antigen:PurifiedHsp70fromPhaseolusaureus(mungbean).HostSpecies:MouseAntibodyClass:IgG1

ThisantibodyrecognizesconstitutiveandinducibleplantHsp70.Itdoesnotcross-reactwithhuman,rat,bacteria(DNAKK)orhumanBIP.

Immunoblotting:useat1ug/ml.Abandof

~70kDaisdetected.

WesternblotdetectionofHsp70inDuckweed(L.minor)cellswith#11138.

ELISA:useat1-10ug/ml.

Thesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.