Anti-Hsp90MonoclonalAntibody
Hsp90andthe94kDaglucose-regulatedprotein,Grp94,aremajormolecularchaperonesofthecytosolandendoplasmicreticulum.Inmammaliancells,thereareatleasttwoHsp90isoforms,Hsp90αandHsp90β,whichareencodedbyseparategenes.AllknownmembersoftheHsp90familyarehighlyconserved,especiallyintheN-terminalandC-terminalregions.Intheabsenceofstress,Hsp90isanessentialcomponentofcellularprocessessuchashormonesignalingandcellcyclecontrol.
Severalregulatoryproteinssuchassteroidreceptors,cellcyclekinasesandp53havebeenidentifiedassubstratesofHsp90.
Thisantibodyrecognizeshuman,mouse,andrabbitHsp90αandHsp90β(90kDa).
BarentRLetal.1998MolEndocrinol12:342;FeltsSJetal.2000JBiolChem275:3305;ArlanderSJetal.2003JBiolChem278:52572.
APPLICATIONSImmunoblotting:useat1-5ug/ml.Abandof
~90kDaisdetected.
DetectionofHsp90intumorcelllinesandratbrainwith
#11098at1ug/ml.
DetectionofHsp90inHeLacelllysatewith#11098at1ug/ml.
Immunohistochemistry:useat1-5ug/ml.Immunoprecipitation:10ugon10ulProteinA-Sepharose+100ulsample.
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:Heat-shockedHeLacelllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

