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Hsp90isahighlyconservedandessentialstressproteinthatisexpressedinalleukaryoticcells.Itparticipatesinthefolding,assembly,maturation,andstABIlizationofspecificproteinsasanintegralcomponentofchaperonecomplexes.Hsp90isrelativelyabundantinunstressedcellsofmostprokaryoticandeukaryoticsystemsandcanbeinducedbyheatshockinsomesystems.Itexistsinadimericformandhasbeenobservedtobindtoseveralothercellularproteinssuchasretroviruskinases,steroidreceptors,hemeregulatedproteinkinase,actin,andtubulin.WhenboundtoATP,Hsp90interactswithco-chaperonescdc37,p23,andvariousimmunophilin-likeproteins,formingcomplexesthatstabilizeandprotecttargetproteinsfromproteasomaldegradation.
Antigen:Hsp90purifiedfromthewatermoldAchlyaambisexualis.
HostSpecies:MouseAntibodyClass:IgG2bPreservatives:0.09%sodiumazideOtheradditives:50%glycerolSPECIFICITYThisantibodyisreactivewithboththeconstitutiveandtheinducIBLeformsofhuman,mouse,rat,rabbit,chicken,Sf9cell,Achlya,andwheatgermHsp90.However,itdoesnotbindtothenativeformofHsp90anddoesnotrecognizeE.coliandyeastHsp90.
Immunoblotting:useat1ug/ml.Abandof
~88kDaisdetected.
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:HeatshockedHeLacelllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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