Hsp60isanabundant,constitutivelyproducedheatshockproteinthatincreasesincellularconcentrationinresponsetocellshock.Itisfoundinmultiplespeciesandisnotablyhomologousinbacteria,plants,andmammals.Hsp60ofPlasmodiumfalciparumisapolypeptideof577aminoacidswithacalculatedmolecularweightof62158Da.TheprimarystructureofP.falciparumhsp60containsaputativemitochondrialtargetingpeptideatitsamino-terminusandaGGMmotifatitscarboxyl-terminus.Theoverallstructureexhibitsstrongconservation(approximately50%)to

thehsp60fromhumanandothereukaryotes,butonlylowhomology(<30%)toarecentlyreportedP.falciparumchaperonin60gene.TheP.falciparumhsp60geneislocatedonchromosome10.Duringheatshock,the

levelofhsp60transcriptinbloodstageparasitesincreasessignificantlyanditsaccumulationcorrelateswiththedurationoftheinduction.

ThisantibodyrecognizesP.falciparumHsp60andcross-reactswithE.coliHsp60/GroEl.

Immunoblotting:useat1ug/ml.Abandof

~62kDaisdetected.

ImmunoblotofPfHsp60in20ugP.falciparum

lysatewith#11146at1ug/ml.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.