PurifiedbyProteinAaffinitychromatography.

TheHsp90proteinchaperoneisahighlyconservedproteincriticalfortheproperfunctioningofvariouscellularsignalingpathwaysandservestomediateappropriatefoldingandprotein-proteininteractions.Hsp90hasbeenshowntobeimportantforthegrowthofinfectiousagentssuchasPlasmodiumfalciparum,thecausativeagentofmalaria.

TreatmentwithgeldanamycineffectivelyinhibitsthegrowthofP.falciparuminculture,presumablythroughitsinteractionwithHsp90.Inaddition,arecentstudyhasshownthatPfHsp90maybemoresensitivetogeldanamycintreatmentthanitshumanhomolog.

ThisantibodyrecognizesP.falciparumHsp90anddoesnotrecognizeHsp90ofhuman,yeast,ordictyostelium..

Immunoblotting:useat1ug/ml.Abandof

~90kDaisdetected.

ImmunoblotofPfHsp90in20ugP.falciparum

lysatewith#11142at0.7ug/ml.

Immunofluorescence:useat20ug/ml.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.