tsp23MonoclonalAntibody
CatalogNo.:11137(clonetsp232A)Size:100uginPBS(1mg/ml),pH7.4,50%glycerol,0.09%sodiumazide.
PurifiedbyProteinGaffinitychromatography.
tsp23(transcriptsimilarp23)isahomologofhumanp23,ahighlyconservedubiquitousproteinandacochaperonefortheHsp90chaperonesystem.Asmallprotein(18-25kDa)withasimplestructure,p23isahighlyacidicphosphoproteinwithanasparticacid-richC-terminaldomain.Althoughnumerousstudieshavefoundp23associatedwithotherproteins(i.e,Festyrosinekinase,hemeregulatedkinaseHRI,Hsf1transcriptionfactor,arylhydrocarbonreceptor,telomerase,andHepadnavirusreversetranscriptase),thefunctionalsignificanceofp23remainstobeclearlydefined.ItappearstobeinvolvedinATP-mediatedHsp90bindingofclientproteins.Arecentstudysuggeststhatp23mightbe
apromisingtargetinleukemicapoptosis.tsp23is44%homologouswithp23.
Antigen:Recombinanttsp23HostSpecies:MouseAntibodyClass:IgG1
SPECIFICITYThisantibodyrecognizeshumanandmousetsp23.Otherspecieshavenotbeeninvestigated.Itdoesnotcross-reactwithp23.
Immunoblotting:useat1ug/ml.Abandof
~19kDaisdetected.
Immunoblotanalysisofextractspreparedfromindicatedmousetissues(Freemanetal.2000Genes&Dev14:433).
Thesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.
Positivecontrol:LysateofHeLacellstransientlytransfectedwithTsp23.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
