ORDERINGINFORMATION
CatalogNo.:26102Format:200ulrabbitantiserumBACKGROUND
ChromograninB(CgB,secretograninI)isasecretorygranulematrixproteinexpressedinawidevarietyofendocrinecellsandneuronswhereitmayhavebothextracellularandintracellularfunctions.ExtracellularrolesforCgBaredependentonitsproteolyticprocessingwithinsecretorygranulestoformsmallerpeptidesthatmayhavearoleintheneuroendocrine/sympathoadrenalstressresponsetosystemicinfection,andCgBfragmentsmayalsohaveanautocrineinhibitoryroleinthereleaseofco-storedhormones,suchasinsulin.
SPECIFICATIONSUMMARY
Antigen:SyntheticpeptidederivedfromtheC-terminus(aa641-655)ofchromograninB(accessionno.NP_001810.Thissequenceisidenticalinhuman,mouse,andratchromograninB.HostSpecies:RabbitSpecificity:Thisantibodyrecognizeshuman,mouse,andratchromograninB.APPLICATIONS
Immunoblotting:usediluted1:2,500-1:10,000.Abandof110kDaisdetected.
AlthoughthepredictedmwofCgBis
~76kDa,thehighlyacidicnatureofCgBcausesittorunatahighermwinSDS-PAGE.
Immunohistochemistry:usediluted1:500-1:5,000.
CgBdetectedinhumanpancreaswithproductno.26102diluted1:1,000.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oCto-70oC.Avoidmultiplefreeze-thaw
cycles.
PRODUCTREFERENCE
ThompsonMEetal.1992.ProlactingranulogenesisisassociatedwithincreasedsecretograninexpressionandaggregationintheGolgiapparatusofGH4C1cells.Endocrinology131:318-326.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
