ORDERINGINFORMATION
CatalogNo.:26108
Format:100ugpeptideaffinity-purifiedrabbitIgG(0.2mg/ml)
BACKGROUND
ChromograninB(CgB,secretograninI)isasecretorygranulematrixproteinexpressedinawidevarietyofendocrinecellsandneuronswhereitmayhavebothextracellularandintracellularfunctions.ExtracellularrolesforCgBaredependentonitsproteolyticprocessingwithinsecretorygranulestoformsmallerpeptidesthatmayhavearoleintheneuroendocrine/sympathoadrenalstressresponsetosystemicinfection,andCgBfragmentsmayalsohaveanautocrineinhibitoryroleinthereleaseofco-storedhormones,suchasinsulin.
SPECIFICATIONSUMMARY
Antigen:SyntheticpeptidederivedfromtheC-terminus(aa641-655)ofCgB(accessionno.NP_001810.Thissequenceisidenticalinhuman,mouse,andratCgB.
HostSpecies:Rabbit
Specificity:Thisantibodyrecognizeshuman,mouse,andratchromograninB.
APPLICATIONS
Immunoblotting:useat0.1-1.0ug/ml
PC12cellextract(ug)2.51.0
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
AlthoughthepredictedmwofCgBis~76kDa,thehighlyacidicnatureofCgBcausesittorunatahighermwinSDS-PAGE.
Immunohistochemistry:useat1-5ug/ml.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oCto-70oC.Avoidmultiplefreeze-thawcycles.
PRODUCTREFERENCE
ThompsonMEetal.1992.ProlactingranulogenesisisassociatedwithincreasedsecretograninexpressionandaggregationintheGolgiapparatusofGh4C1cells.Endocrinology131:318-326.Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
