Digoxigenin(DIG)isasteroidfoundexclusivelyintheflowersandleavesoftheplantsDigitalispurpurea,DigitalisorientalisandDigitalislanata(foxgloves).ItisusedinmanymolecularBIOLOGyapplicationssimilarlytootherpopularhaptenssuchasDNP(dinitrophenol),biotin,andfluoresceinandisoftenusedasanimmunohistochemicalMarkerforinsituhybridization.InthiscaseitisconjugatedtoasinglespeciesofRNAnucleotidetriphosphate(typicallyuridine),whichisthenincorporatedintoRNAasitissynthesized.

Thisantibodyreactswithfreeandbounddigoxigenin.

ELISA:useat0.1-1.0ug/ml.Immunoblotting:useat2-5ug/ml.Immunohistochemistry:Useat1-5ug/ml.Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Reconstituteindistilledwater.

Thisproductisstableforatleastone(1)yearat-20oCto-70oC.Reconstitutedproductshouldbestoredinappropriatealiquotstoavoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.