CatalogNo.:19401thru19404DiluteinPBSormediumthatisidenticalSize:100ugProteinG-purifiedtothatusedintheassaysystemantibodyinPBS,pH7.
STORAGEANDSTABILITYSPECIFICATIONSUMMARYTheseantibodiesarestableforatleastAntigen:Humanendothelin-1(ET-1).one(1)yearat-20oCto-70oC.StoreHostSpecies:Mouseproductinappropriatealiquotstoavoid
AntibodyClass:IgG1multiplefreeze-thawcycles.
CloneNos.:19401=20-2101.70
19402=20-2111.41
19403=20-2125.4
19404=20-2201.1
a
AntibodyConcentrations(pg/tube)for50%B/B0Inthepresenceof:19401194021940319404Endothelin-12x1022x1011x1018x101
Endothelin-22x1022x1004x1008x101
Endothelin-32x1041x1031x1032x103
BigEndothelin0*2x1004x1000*EndothelinC-terminalhexapeptide0*0*
Atrialnatriureticpeptide0*0*
SarafotoxinS6c2x104
AngiotensinI0*
AngiotensinII0*
*Nodisplacementwithantibodyat105pg/tube.
Immunohistochemistry:ELISA:useat5-20ug/mlwithendothelin-1onthesolidphase.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
Paraffin-embeddedratkidneyexposedto60minutesofischemia.ET-1expressionisseenintheendotheliumoftheperitubularcapillarynetwork(arrows,darkreactionproduct)with
#19401at5-20ug/ml.WilhelmSMetal.1999KidneyInternational55:1011-1018.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticapplications.
