ORDERINGINFORMATION

CatalogNo.:50116CloneNo.:OCG3Size:100ug

Format:Antibodywaspurifiedbycolumnchromatography,dissolvedin20mMTBS,pH7.5,containing1.0%bovineserumalbumin,andthenlyophilized.Thelyophilizedantibodydoesnotcontainpreservative.

BACKGROUND

Osteocalcinisanoncollagenous,49aminoacidglutamate-richpolypeptidebonematrixproteinwithamolecularweightofabout5800Da.Osteoblastsproduceosteocalcinandincorporateitintothebonematrix.Osteocalcinisreleasedintothecirculationfromthematrixduringboneresorptionand,therefore,isconsideredaMarkerofboneturnoverratherthanaspecificmarkerofboneformation.

SPECIFICATIONSUMMARY

Antigen:BovineosteocalcinHostSpecies:MouseAntibodySubtype:IgG3

Specificity:Epitopewithinresidues21-31;human,bovine,rat,rabbit,pig,goat,andsheep.

APPLICATIONS

SandwichELISA:1-10ug/mlwithimmobilizedantigen,pairwithcatalogno.50120.

Immunoblotting:1-10ug/ml,reducingornon-reducingconditions.

Immunohistochemistry:1-10ug/ml,frozenorparaffin-embeddedtissueafterproteinaseK(0.4m/ml)antigenretrieval.

ChondromaSynovialSarcomaSynovialSarcoma

Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

Dissolvelyophilizedantibodyin50ulofdistilledwater(2.0mg/mlfinalconcentration)thendilutefurtherforapplicationsinPBSormediathatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Storestocksolution(2.0mg/ml)inaliquotsat-20℃for1year,orat4℃for6monthsafteradding0.1%sodiumazide.Avoidrepeatedfreeze-thawcycles.Dilutedantibodyshouldnotbestored.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.