ORDERINGINFORMATION

CatalogNo.:50120CloneNo.:OCG4Size:100ug

Format:Antibodywaspurifiedbycolumnchromatography,dissolvedin10mMPBS,pH7.4,containing1.0%bovineserumalbumin,andthenlyophilized.Thelyophilizedantibodydoesnotcontainpreservative.

BACKGROUND

Osteocalcinisanoncollagenous,49aminoacidglutamate-richpolypeptidebonematrixproteinwithamolecularweightofabout5800Da.Osteoblastsproduceosteocalcinandincorporateitintothebonematrix.Osteocalcinisreleasedintothecirculationfromthematrixduringboneresorptionand,therefore,isconsideredaMarkerofboneturnoverratherthanaspecificmarkerofboneformation.

SPECIFICATIONSUMMARY

Antigen:BovineosteocalcinHostSpecies:MouseAntibodySubtype:IgG1

Specificity:Epitopewithinresidues4-9;human,bovine,rabbit,pig,dog,goat,sheep,andchicken.

APPLICATIONS

SandwichELISA:1-10ug/mlwithimmobilizedantigen,pairwithcatalogno.50116.

Immunoblotting:1-10ug/ml,reducingornon-reducingconditions.

Immunohistochemistry:1-10ug/ml,frozenorparaffin-embeddedtissueafterproteinaseK(0.4m/ml)antigenretrieval.

ChondromaSynovialSarcomaSynovialSarcoma

Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

Dissolvelyophilizedantibodyin50ulofdistilledwater(2.0mg/mlfinalconcentration)thendilutefurtherforapplicationsinPBSormediathatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Storestocksolution(2.0mg/ml)inaliquotsat-20℃for1year,orat4℃for6monthsafteradding0.1%sodiumazide.Avoidrepeatedfreeze-thawcycles.Dilutedantibodyshouldnotbestored.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.