ORDERINGINFORMATION
CatalogNo.:26101Format:200ulrabbitantiserumBACKGROUND
SecretograninII(SgII),encodedbythegeneSCG2,isamemberofthegraninfamilyofneuroendocrinesecretoryproteins.ThegraninfamilyalsoincludeschromograninA(CgA),chromograninB(CgB),andseveralothersecretogranin-likeproteins.Studiesinrodentssuggestthatthefull-lengthprotein,SgII,isinvolvedinthepackagingorsortingofpeptidehormonesandneuropeptidesintosecretoryvesicles.SgIIoccursinavarietyofneoplasmsarisingfromendocrineandneuroendocrinecellsandcanbeausefulhistologicaltumorMarkerforthosecells.
SPECIFICATIONSUMMARY
Antigen:SyntheticpeptidederivedfromtheN-terminusofmatureSgIIprotein:QRNQLLGKEPDLRLENVQK(Accessionno.AAA36607).
HostSpecies:RabbitSpecificity:Thisantibodyrecognizeshuman,rat,andsquirrelmonkeySgII.APPLICATIONS
Immunoblotting:usediluted1:2,500-1:10,000.Abandof~86kDaisdetected.AlthoughthepredictedmwofSgIIis~71kDa,thehighlyacidicnatureofSgIIcausesittorunatahighermwinSDS-PAGE.
Immunohistochemistry:usediluted1:1,000.
Immunoprecipitation:usediluted1:100-1:500.
Positivecontrol:ratPC12adrenalpheochromocytomacells
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisantibodyisstableforatleastone(1)yearat-20oCto-70oC.Avoidrepeatedfreeze-thawcycles.
PRODUCTREFERENCES
ThompsonMEetal.1992.ProlactingranulogenesisisassociatedwithincreasedsecretograninexpressionandaggregationintheGolgiapparatusofGHvC1cells.Endocrinology131:318-326.
VasauskasAAetal.2011.RegulationanddistributionofsquirrelmonkeychorionicgonadotropinandsecretograninIIinthepituitary.GeneralandComparativeEndocrinology170:509-513.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
