ORDERINGINFORMATION

CatalogNo.:26107

Format:100ugpeptideaffinity-purifiedrabbitIgG(2mg/ml)

BACKGROUND

SecretograninII(SgII),encodedbythegeneSCG2,isamemberofthegraninfamilyofneuroendocrinesecretoryproteins.ThegraninfamilyalsoincludeschromograninA(CgA),chromograninB(CgB),andseveralothersecretogranin-likeproteins.Studiesinrodentssuggestthatthefull-lengthprotein,SgII,isinvolvedinthepackagingorsortingofpeptidehormonesandneuropeptidesintosecretoryvesicles.SgIIoccursinavarietyofneoplasmsarisingfromendocrineandneuroendocrinecellsandcanbeausefulhistologicaltumorMarkerforthosecells.

SPECIFICATIONSUMMARY

Antigen:SyntheticpeptidederivedfromtheN-terminusofmatureSgIIprotein:

QRNQLLGKEPDLRLENVQK(Accessionno.AAA36607).

HostSpecies:Rabbit

Specificity:Thisantibodyrecognizeshuman,rat,andsquirrelmonkeySgII.

APPLICATIONS

Immunoblotting:useat0.5-1.0ug/ml.

Immunohistochemistry:useat2-5ug/ml.

86,000

PC12extract(1ug)

AlthoughthepredictedmwofSgIIis~71kDa,thehighlyacidicnatureofSgIIcausesittorunatahighermwinSDS-PAGE.

Squirrelmonkeypituitarygland,stainingvisualizedwithTexasRed-labeledgoatanti-rabbitantibody.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oCto-70oC.Avoidrepeatedfreeze-thawcycles.

PRODUCTREFERENCE

VasauskasAAetal.2011.RegulationanddistributionofsquirrelmonkeychorionicgonadotropinandsecretograninIIinthepituitary.GeneralandComparativeEndocrinology170:509-513.

Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.