productdatasheet
CatalogNo.:1012
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychroma-tography.
Humanimmunodeficiencyvirus(HIV)andrelatedvirusesrequirecoreceptorsinadditiontoCD4toinfecttargetcells.SomeGprotein-coupledreceptorsinthechemokinereceptorfamily,includingCCR5,CXCR4,CCR3,CCR2b,andCCR8inthechemokinereceptorfamily,havebeenidentifiedasHIVcoreceptors.CXCR4(alsocalledFusin,LESTR,HUMSTR)isaprincipalcoreceptorforT-celltropicstrainsofHIV-1.CXCR4isalsorequiredfortheinfectionbydual-tropicstrainsofHIV-1andmediatesCD4-independentinfectionbyHIV-2.Theα-chemokineSDF-1istheligandforEXCR4andpreventsinfectionbyT-celltropicHIV-1.CXCR4associateswiththesurfaceCD4-gp120complexbeforeHIVenterstargetcells.Theamino-terminaldomainandthesecondextracellularloopofCXCR4serveasHIVbindingsites.
CXCR4messengerRNAlevelscorrelatewithHIV-1permissivenessindiversehumancelltypes.AntibodiestoCXCR4mayblockHIV-1andHIV-2infectionofhumantargetcells.
Antigen:Peptidecorrespondingtoaa182-196inthesecondextracellularloop(EL)ofhumanCXCR4.
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizeshumanandmouseCXCR4(40kD).
Immunoblotting:useat1:500-1:1,000dilution.
Positivecontrol:WholecelllysatefromHeLacells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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