ThenonstructuralregulatoryTaxproteinsareuniquecharacteristicsofdeltaretroviruses,asubgroupofretroviruses,whichconferslong-termpersistentinfectiontomammalianlymphocytes.Theseproteinsarecrucialforproductiveviralreplication,andtheystimulatetheproliferationofhostlymphocyticcells.TaxhasbeenshowntobeoncogenicbecauseittransformsandimmortalizesrodentfibroblastsandhumanT-lymphocytes.Taxalsostimulatescellgrowthbydirectbindingtocyclin-dependentkinaseholenzymesand/orinactivatingtumorsuppressors(e.g.p53,DLG).Moreover,Taxsilencescellularcheckpoints,whichguardagainstDNAstructuraldamageandchromosomalmissegregation,therebyfavoringthemanifestationofamutatorphenotypeincells.

ThisantibodyreactswithHTLV-1Taxexpressedintransientlytransfectedcells.

ELISA:useat0.1-1.0ug/ml.Immunoblotting:useat2-10ug/ml.Abandof~42kDaisdetected.

Immunohistochemistry:useat2-20ug/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Reconstituteindistilledwater.

Thisproductisstableforatleastone(1)yearat-20oCto-70oC.Reconstitutedproductshouldbestoredinappropriatealiquotstoavoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.