ORDERINGINFORMATION
CatalogNos.:
22001cloneXI64IgM22002cloneXI126IgG2b
22003cloneXIR278IgG1Discontinued
22004cloneXIR1323IgM
Format:200uginPBS,pH7.4.
SPECIFICATIONSUMMARY
Antigenfor22001and22002:heat-killedS.pneumoniaestrainR36A.
Antigenfor22004:full-lengthrecombinantpneumococcalsurfaceproteinA(PspA)type2strainD39.
Hostspecies:Mouse
Specificity:TheseantibodiesrecognizepneumococcalsurfaceproteinA.
APPLICATIONS
ELISA:CatalogNo.Titer220011:16,000
220021:64,000
220041:32,000
ELISAisanindirectassayagainstStrep.pneumoniaecellsonthesolidphase.Titrationsstartwithantibodiesat1mg/ml.
ReactivityofmonoclonalantibodiesspecificforPspAwithclinicalisolatesofStreptococcuspneumoniae | |||
CapsularSEROtype | No.tested | MAb #22001 | MAb #22002 |
3 | 10 | 5 | 5 |
4 | 11 | 1 | 4 |
6 | 22 | 4 | 7 |
7 | 1 | 0 | 0 |
9 | 3 | 0 | 3 |
14 | 3 | 0 | 0 |
16 | 1 | 0 | 0 |
19 | 3 | 0 | 0 |
23 | 3 | 1 | 1 |
ReactivityofmonoclonalantibodiesspecificforPspAwithclinicalisolatesofStreptococcuspneumoniae | |||
Capsularserotype | No.tested | MAb #22001 | MAb #22002 |
3 | 10 | 5 | 5 |
4 | 11 | 1 | 4 |
6 | 22 | 4 | 7 |
7 | 1 | 0 | 0 |
9 | 3 | 0 | 3 |
14 | 3 | 0 | 0 |
16 | 1 | 0 | 0 |
19 | 3 | 0 | 0 |
23 | 3 | 1 | 1 |
Immunoblotting:
Seereference3below.
ImmunoblotofrecombinantpneumococcalsurfaceproteinA(PspA)reactedwith#22002.Lanes:1and2,phagelysatefromfull-lengthPspA+clonewithandwithoutinductionbyisopropyl-β-D-thiogalactopyranoside(IPTG),respectively;3,cellwallextractfrompneumococcaltype2strainD39.
Seereference4below.
Invivoprotection:Theseantibodiesprotectagainstlethalpneumococcalinfectioninamousemodel.Seereference1below.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Theseantibodiesarestableforatleastone(1)yearfromdateofreceiptat-20oCto-70oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.
PRODUCTREFERENCES
McDanielL.etal.1984Monoclonalantibodiesagainstprotease-sensitivepneumococcalantigenscanprotectmicefromfatalinfectionwithStreptococcuspneumoniae.JExpMed160:386-397.
McDanielL.etal.1987Aprotectivemonoclonalantibodythatreactswithanovelantigenofpneumococcalteichoicacid.MicrobialPathogenesis3:249-260.
CrainMJetal.1990PneumococcalsurfaceproteinA(PspA)isserologicallyhighlyvariableandisexpressedbyallclinicallyimportantcapsularserotypesofStreptococcuspneumoniae.InfectionandImmunity58:3293-3299.
McDanielL.etal.1991PspA,AsurfaceproteinofStreptococcuspneumoniae,iscapableofelicitingprotectionagainstPneumococciofmorethanonecapsulartype.InfectionandImmunity59:222-228.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
