Library Pack No. 100ug/clone

180101 All 4 clones

Library Pack No. 100ug/clone

180101 All 4 clones

ORDERING INFORMATION

Catalog No. Clone No. MAb Subtype Size

18001 TGEV10-116.3.3 IgG1 100ug, 500ug

18002 TGEV11-314.6 IgG1 100ug, 500ug

18003 TGEV15-886.6 IgG2a 100ug, 500ug

18004 TGEV15-948.2.1 IgG2a 100ug, 500ug

Format: Purified antibody in PBS, pH 7.4

BACKGROUND

Transmissible Gastroenteritis (TGEV) is an enveloped Coronavirus with a positive-sense single- stranded RNA genome and a helical symmetry. The genomic size of coronaviruses is approximately 28.6 kilobases. Proteins that contribute to the overall structure of TGEV include the spike (S), envelope (E), membrane (M) and nucleocapsid (N). Other coronaviruses belonging to this family include Feline coronavirus (FeCV), Canine coronavirus (CCV), and Feline infectious peritonitis virus (FIPV). TGEV infects pigs. However, in young pigs, the mortality rate is close to 100%. The pathology of TGEV is similar to that of other coronaviruses. Coronaviruses enter the host by first attaching to the host cell using the spike glycoprotein. The S protein interacts with the porcine aminopeptidase N (pAPN), a cellular receptor, to aide in its entry. A domain in the S spike protein is recognized by pAPN, and transfection of pAPN occurs to nonpermissive cells and infects them with TGEV. Once the virus infects the host, it multiplies in the cell lining of the small intestine resulting in the loss of absorptive cells that in turn leads to shortening of villi. The infected swine then has reduced capability for digesting food and die from dehydration.

SPECIFICATION SUMMARY

Antigen: TGEV-infected ST cells

Host Species: Mouse

Specificity: These antibodies recognize TGEV and do not cross-react with FIPV, FeCV, or CCV.

#18003 and #18004 can neutralize TGEV in vitro.

APPLICATIONS

These antibodies have been qualified for use in ELISA to detect TGEV. #18003 and #18004 may be used in TGEV neutralization assays. Endusers should determine optimal concentrations for their applications.

DILUTION INSTRUCTIONS

Dilute in PBS or medium that is identical to that used in the assay system.

STORAGE AND STABILITY

These antibodies are stable for at least one (1) year at -20o to -70oC. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.

For in vitro investigational use only. Not intended for use in therapeutic or diagnostic procedures.