Library Pack No. 100ug/clone
180101 All 4 clones
Library Pack No. 100ug/clone
180101 All 4 clones
ORDERING INFORMATION
Catalog No. Clone No. MAb Subtype Size
18001 TGEV10-116.3.3 IgG1 100ug, 500ug
18002 TGEV11-314.6 IgG1 100ug, 500ug
18003 TGEV15-886.6 IgG2a 100ug, 500ug
18004 TGEV15-948.2.1 IgG2a 100ug, 500ug
Format: Purified antibody in PBS, pH 7.4
BACKGROUND
Transmissible Gastroenteritis (TGEV) is an enveloped Coronavirus with a positive-sense single- stranded RNA genome and a helical symmetry. The genomic size of coronaviruses is approximately 28.6 kilobases. Proteins that contribute to the overall structure of TGEV include the spike (S), envelope (E), membrane (M) and nucleocapsid (N). Other coronaviruses belonging to this family include Feline coronavirus (FeCV), Canine coronavirus (CCV), and Feline infectious peritonitis virus (FIPV). TGEV infects pigs. However, in young pigs, the mortality rate is close to 100%. The pathology of TGEV is similar to that of other coronaviruses. Coronaviruses enter the host by first attaching to the host cell using the spike glycoprotein. The S protein interacts with the porcine aminopeptidase N (pAPN), a cellular receptor, to aide in its entry. A domain in the S spike protein is recognized by pAPN, and transfection of pAPN occurs to nonpermissive cells and infects them with TGEV. Once the virus infects the host, it multiplies in the cell lining of the small intestine resulting in the loss of absorptive cells that in turn leads to shortening of villi. The infected swine then has reduced capability for digesting food and die from dehydration.
SPECIFICATION SUMMARY
Antigen: TGEV-infected ST cells
Host Species: Mouse
Specificity: These antibodies recognize TGEV and do not cross-react with FIPV, FeCV, or CCV.
#18003 and #18004 can neutralize TGEV in vitro.
APPLICATIONS
These antibodies have been qualified for use in ELISA to detect TGEV. #18003 and #18004 may be used in TGEV neutralization assays. Endusers should determine optimal concentrations for their applications.
DILUTION INSTRUCTIONS
Dilute in PBS or medium that is identical to that used in the assay system.
STORAGE AND STABILITY
These antibodies are stable for at least one (1) year at -20o to -70oC. Store product in appropriate aliquots to avoid multiple freeze-thaw cycles.
For in vitro investigational use only. Not intended for use in therapeutic or diagnostic procedures.