Acidsensing(proton-gated)ionchannel1(ASIC1)isacationchannelwithhighaffinityforsodiumwhichisgatedbyextracellularprotonsandinhibitedbythediureticamiloride.ItisalsopermeableforCa(2+),Li(+)andK(+)andmediatesglutamate-independentCa(2+)entryintoneuronsuponacidosis.ThisCa(2+)overloADIngistoxicforcorticalneuronsandmaybeinpartresponsIBLeforischemicbraininjury.ASIC1

functionsasapostsynapticprotonreceptorthatinfluencesintracellularCa(2+)concentrationandcalmodulin-dependentproteinkinaseIIphosphory-lation.

ThisantibodyrecognizesmouseandratASIC1.Itdoesnotcross-reactwithASIC2.

Immunoblotting:useat1ug/ml.Abandof

~60kDaisdetected.

Positivecontrol:Ratbrainlysate.

Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.