productdatasheet
Acetylationisacommonpost-translationalmodificationoflysine.Theconservedamino-terminaldomainsofthefourcorehistones(H2A,H2B,H3,andH4)containlysinesthatareacetylatedbyhistoneacetyltransferases(HATs)anddeacetylatedbyhistonedeacetylases(HDACs).Post-translationalacetylationanddeacetylationoflysinehavebeenrecognizedasintracellularsignalsthatarecriticalinregulatinggenetranscription,cellcycleprogression,apoptosis,DNArepair,andcytoskeletalorganization.Regulationofproteinacetylationisimpairedinsomecancersandpolyglutaminediseases,andHDACsarepromisingtargetsforanti-cancerdrugscurrentlyunderinvestigation.
Thisantibodyrecognizesproteinscontainingacetylatedlysineresidues.Itdoesnotreactwithnon-acetylatedlysineresidues.
Immunoblotting:useat1ug/ml.
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:Acetylatedchickenerythrocytehistones.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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