CatalogNo.:56498

Size:400ulpurifiedrabbitimmunoglobulin(250ug/ml)inphosphate-bufferedsaline,pH

7.0and50%glycerol.

Post-translationalmodificationsofproteinsplaycriticalrolesintheregulationandfunctionofmanyBIOLOGicalprocesses.Acommonpost-transcriptionalmodificationoflysineisacetylation.Theconservedamino-terminaldomainsofthefourcorehistones(H2A,H2B,H3,andH4)containlysinesthatareacetylatedbyhistoneacetyltransferases(HATs)anddeacetylatedbyhistonedeacetylases(HDACs).Regulationofproteinacetylationstatusisimpairedincancerandpolyglutaminediseases,andHDACshavebecomepromisingtargetsforanti-cancerdrugscurrentlyindevelopment.

Antigen:Acetylatedlysine-KLHconjugate.

HostSpecies:RabbitAntibodyClass:PolyclonalPreservatives:None.

Thisantibodyrecognizesproteinscontainingacetylatedlysineresidues.Itdoesnotrecognizenon-acetylatedproteins.

Immunoblotting:useat1-2ug/ml

WesternblotanalysisofacetylatedhistonefromTSA-treatedmousespleencells.

ELISA:useat1-10ug/mlwithacetylatedlysine-containingproteinsonthesolidphase.

Immunoprecipitation

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisproductisstableforatleast1yearat

-20oC.Freezeinmultiplealiquotstoavoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.