ORDERINGINFORMATION

Catalogno.:56583(clone343-26)

Format:100ug(1mg/ml)ProteinG-purifiedantibodyinPBS,pH7.4,0.1%sodiumazide,50%glycerol.

BACKGROUND

ATF4belongstothelargeATF/CREBfamilyoftranscriptionfactors.TheseproteinsbindDNAviatheirbasicregionanddimerizeviatheirleucinezipperdomaintoformavarietyofhomo-andheterodimersthatregulategenetranscription.Twoalternativetranscriptsencodingthesameproteinhavebeendescribed.ATF4transcriptionfactorisalsoknowntoplayroleinosteoblastdifferentiation.Terminalosteoblastdifferentiation,issignificantlyinhibitedbytheinactivationofJNK;JNKinactivationdownregulatesexpressionofATF-4.

SPECIFICATIONSUMMARY

Antigen:Fusionproteincorrespondingtoaa25-327ofhumanATF4.Thissequenceis86%identicalinrat,85%identicalinmouse,and<50%identicalinATF5.

Accessionnos:NP_001666.2,P18848

GeneID:468

HostSpecies:Mouse

AntibodyClass:IgG2a

Specificity:ThisantibodyrecognizeshumanandratATF4.

APPLICATIONS

Immunoblotting:useat1-5ug/ml.Abandof

~60kDaisdetected.

Immunofluorescence:useat10ug/ml.

DetectionofATF4inratbrainlysatewith#56583.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.

DetectionofATF4inneuroblastomacelllineSK-N-BEwith#56583at10ug/ml:DAPI(blue)nuclearstain,TexasRedFactinstain,ATTO488(green)ATF4stain.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisproductisstableforatleastone(1)yearat-20oC.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.