productdatasheet
CatalogNo.:56467(cloneS95-35)Size:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.
GABAisthemajorinhibitoryneuro-transmitterinthevertebratebrain.GABAreceptorsconsistoffivesubunitsthatformachlorideionchannel.Subunitfamiliesα,β,γ,andδhavebeenstudiedextensively;πandεhavebeenidentifiedrecently.
StudiesofrecombinantGABAreceptorshaveshownthatindividualsubunitsandtheirsubtypesconferdifferentsensitivitiestoGABAreceptormodulators.SubunitsubtypesaredifferentiallyexpressedthroughoutdevelopmentandindifferentCNSregions,reducingthetotalnumberofpossIBLeisoformsthatcanbeformedindifferentbrainregionsandinindividualcells.
SPECIFICATIONSUMMARYAntigen:Fusionproteincorrespondingtoaa355-394ofmouseAlpha1GABA-Areceptor(accessionno.P62812).HostSpecies:MouseAntibodyClass:IgG2aThisantibodyrecognizeshuman,mouse,andratAlpha1GABA-Areceptor.Itdoesnotcross-reactwithAlpha2orAlpha3GABA-Areceptors.
APPLICATIONSImmunoblotting:useat1-10ug/ml.Abandof~55kDaisdetected.
Adultratbrainmembrane(left)andtransiently-transfectedCOScellextracts(right)probedwith
#56467
ImmunohistochemistryandImmunocytochemistry:useat0.1-1ug/mlImmunofluorescence:useat1-10ug/mlThesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:Ratbrainlysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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