AMIGO-1MonoclonalAntibody
PurifiedbyProteinGaffinitychromatography.
Theamphoterin-inducedgeneandORF(AMIGO)familyofproteinsconsistsofAMIGO-1,AMIGO-2andAMIGO-3.All
threemembersaresinglepasstypeImembraneproteinsthatcontainseveralleucine-richrepeats,oneIgGdomainandatransmembranedomain.TheAMIGOproteinsarespecificallyexpressedonfibertractsofneuronaltissuesandparticipateintheirformation.Theycanformcomplexeswitheachother,butcanalsoself-bind.AMIGO1,(alsodesignatedAlivin2)promotesgrowthandfasciculationofneuritesandplaysaroleinmyelinationandfasciculationofdevelopingneuralaxons.
Antigen:Fusionproteincorrespondingtoaa554-574,thecytoplasmicC-terminus,ofhumanAMIGO-1(accessionno.Q86WK6).
HostSpecies:MouseAntibodyClass:IgG1SPECIFICITYThisantibodyrecognizeshuman,mouseandratAMIGO-1.Itdoesnotcross-reactwithAMIGO-2orAMIGO-3.
Immunoblotting:useat1ug/ml.Predictedmolecularweightis~60-80kDadependingonmaturity/glycosylation.
#56530onratbrainandmousebrainlysates.
PositiveControl:ratbrainlysate.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
