AmyloidBeta1-16(Aβ1-16)MonoclonalAntibody
CatalogNo.:57004(cloneAb5)Size:100uginPBS,pH7.4.PurifiedbyProteinGaffinitychromatography.
Accumulationandaggregationofamyloidβ(Aβ)inthebrainisindicatedasthetriggerofapathologicalcascadethatcausesAlzheimerdisease(AD).ThereisnowcompellingevidencethatmetalbindingtoAβisinvolvedinADpathogenesis.Theaminoacidregion1-16iswidelyconsideredasthemetalbindingdomainofAβ.Unlikecopper(II)thatpreferstheN-terminalaminogroupasthemainbindingsite,zinc(II)ispreferentiallyplacedinthe8-16aminoacidicregionofAβ(1-16).
Antigen:AggregatedAβ1-42,fibrillarAβ.
HostSpecies:Mouse
AntibodyClass:IgG2b
ThisantibodyrecognizesanepitopewithinAβ1-16aswellasotherAβpeptides:Aβ37,Aβ38,Aβ39,Aβ40,andAβ42.NOTE:WhenadmiNISTeredtoyoungTg2576micewithminimalAβdepositionandtooldermicewithhigherAβloads,thisantibodyreducedAβaccumulationinthebrain.
APPLICATIONSImmunoblotting,Immunohistochemistry:Immunofluorescence,Immunoprecipitation,Testat1-10ug/mlinallapplications.
SandwichELISAascaptureorHRP-conjugateddetectionantibody.
Thesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.
SandwichELISAprotocolonnextpage.Seespecificproductreferencesbelowformoreinformation.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.
LevitesYetal.2006.Anti-Aβ42andAnti-Aβ40specificmonoclonalantibodiesattenuateamyloiddepositioninanAlzheimer’sdiseasemousemodel.JClinInvest116:193-201.
LevitesYetal.2006.IntracranialAdeno-AssociatedVirus-MediatedDeliveryofAnti-PanAmyloidβ,Amyloidβ40,andAmyloidβ42Single-ChainVariableFragmentsAttenuatesPlaquePathologyinAmyloidPrecursorProteinMice.JNeurosci26:11923-11928.
LevitesYetal.2006.Insightsintothemechanismsofactionofanti-AβantibodiesinAlzheimer’s
diseasemousemodels.FASEBJ20:2576-8.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
SandwichELISAProtocol
96-wellELISAplatesarecoatedwithcaptureMAbat2.5-5ug/wellandincubatedat4oCovernight.Thenextday300ulofblockingbufferisadded,andplatesareagainincubatedat4oCovernight.ThenextdayplatesarewashedinPBSandserialdilutionofAβsamplesareadded;platesareincubatedovernightat4oC.ThenextdayplatesarewashedinPBS,andHRP-conjugateddetectionAβMAbisadded;platesareincubatedfor4hrsatroomtemperature.PlatesarewashedinPBS-TweenanddevelopedwithTMBsubstratefor5
mins.
