AmyloidBeta1-40(Aβ40)MonoclonalAntibody
CatalogNo.:57002(cloneAb40.1)Size:100uginPBS,pH7.4.PurifiedbyProteinGaffinitychromatography.
Accumulationandaggregationofamyloidβ(Aβ)inthebrainisindicatedasthetriggerofapathologicalcascadethatcausesAlzheimerdisease(AD).Thehighlyamyloidogenic42-aminoacidformofAβ(Aβ42)andaminoterminallytruncatedformsofAβ(Aβx-42)arethepredominantspeciesofAβtypicallyfoundindiffuseandsenileplaqueswithintheADbrain.ThereisastrongcorrelationbetweenAβ40positivityandmatureplaques.IntheADcortex,diffuseplaques,representingtheearlieststageofAβdeposition,arepositiveforAβ42,butnegativeforAβ40.
Antigen:Syntheticpeptidecorrespond-ingtoAβ35-40.
HostSpecies:Mouse
AntibodyClass:IgG1
ThisantibodyspecificallyrecognizesanepitopewithinAβx-40.NOTE:WhenadmiNISTeredtoyoungTg2576micewithminimalAβdeposition,thisantibodyreducedAβaccumulationinthebrain.
APPLICATIONSImmunoblotting,Immunohistochemistry:Immunofluorescence,Immunoprecipitation,Testat1-10ug/ml.
SandwichELISA(worksequallywellascaptureordetectionantibody).Thesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.
SandwichELISAprotocolonnextpage.Seespecificproductreferencesbelowformoreinformation.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.
LevitesYetal.2006.Anti-Aβ42andAnti-Aβ40specificmonoclonalantibodiesattenuateamyloiddepositioninanAlzheimer’sdiseasemousemodel.JClinInvest116:193-201.
LevitesYetal.2006.IntracranialAdeno-AssociatedVirus-MediatedDeliveryofAnti-PanAmyloidβ,Amyloidβ40,andAmyloidβ42Single-ChainVariableFragmentsAttenuatesPlaquePathologyinAmyloidPrecursorProteinMice.JNeurosci26:11923-11928.
LevitesYetal.2006.Insightsintothemechanismsofactionofanti-AβantibodiesinAlzheimer’sdiseasemousemodels.FASEBJ20:2576-8.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
SandwichELISAProtocol
96-wellELISAplates(ImmulonHBX4)arecoatedwithcaptureMAbat2.5-5ug/wellandincubatedat4oCovernight.Thenextday300ulofblockingbufferisadded,andplatesareagainincubatedat4oCovernight.ThenextdayplatesarewashedinPBSandserialdilutionofAβsamplesareadded;platesareincubatedovernightat4oC.ThenextdayplatesarewashedinPBS,andHRP-conjugateddetectionAβMAbisadded;platesareincubatedfor4hrsatroomtemperature.PlatesarewashedinPBS-TweenanddevelopedwithTMBsubstratefor5mins.
