AmyloidBeta1-42(Aβ42)MonoclonalAntibody
CatalogNo.:57001(cloneAb42.2)Size:100uginPBS,pH7.4.PurifiedbyProteinGaffinitychromatography.
Accumulationandaggregationofamyloidβ(Aβ)inthebrainisindicatedasthetriggerofapathologicalcascadethatcausesAlzheimerdisease(AD).Thehighlyamyloidogenic42-aminoacidformofAβ(Aβ42)andaminoterminallytruncatedformsofAβ(Aβx-42)arethepredominantspeciesofAβtypicallyfoundindiffuseandsenileplaqueswithintheADbrain.TherelativelevelsofAβ42appeartobethekeyregulatorsofAβaggregationintoamyloid;thusAβ42hasbeenimplicatedastheinitiatingmoleculeinthepathogenesisofAD.
Antigen:Syntheticpeptidecorrespond-ingtoAβ35-42.
HostSpecies:Mouse
AntibodyClass:IgG1
ThisantibodyspecificallyrecognizesanepitopewithinAβx-42.NOTE:WhenadmiNISTeredtoyoungTg2576micewithminimalAβdeposition,thisantibodyreducedAβaccumulationinthebrain.
APPLICATIONSImmunoblotting,Immunohistochemistry:Immunofluorescence,ImmunoprecipitationTestat1-10ug/ml.
SandwichELISA(ascaptureantibodywith
#57004asHRP-conjugateddetectionantibody).Thesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.
SandwichELISAprotocolonnextpage.Seespecificproductreferencesbelowformoreinformation.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.
LevitesYetal.2006.Anti-Aβ42andAnti-Aβ40specificmonoclonalantibodiesattenuateamyloiddepositioninanAlzheimer’sdiseasemousemodel.JClinInvest116:193-201.
LevitesYetal.2006.IntracranialAdeno-AssociatedVirus-MediatedDeliveryofAnti-PanAmyloidβ,Amyloidβ40,andAmyloidβ42Single-ChainVariableFragmentsAttenuatesPlaquePathologyinAmyloidPrecursorProteinMice.JNeurosci26:11923-11928.
LevitesYetal.2006.Insightsintothemechanismsofactionofanti-AβantibodiesinAlzheimer’sdiseasemousemodels.FASEBJ20:2576-8.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
SandwichELISAProtocol
96-wellELISAplatesarecoatedwithcaptureMAbat2.5-5ug/wellandincubatedat4oCovernight.Thenextday300ulofblockingbufferisadded,andplatesareagainincubatedat4oCovernight.ThenextdayplatesarewashedinPBSandserialdilutionofAβsamplesareadded;platesareincubatedovernightat4oC.ThenextdayplatesarewashedinPBS,andHRP-conjugateddetectionAβMAbisadded;platesareincubatedfor4hrsatroomtemperature.PlatesarewashedinPBS-TweenanddevelopedwithTMBsubstratefor5
mins.
