CatalogNo.:57005

Size:100ulpurifiedrabbitimmunoglobulininphosphate-bufferedsaline,pH7.0,0.09%sodiumazideand50%glycerol.

Amyloidmonomericproteinscanoligomerizeintodestructiveamyloidfibrils.Amyloidogenicconformationsofnon-diseaserelatedproteinscanbecreatedbypartialproteinmisfoldingordenaturation.Manydegenerativediseasesareknowntoberelatedtotheaccumulationofmisfoldedproteinsasamyloidfibers.Theseincludetheamyloid-βpeptideplaquesandtauneurofibrillarytanglesinsenileplaquesofAlzheimer’ssymptomology,thedepositionofα-synucleinintheLewybodiesofParkinson’sdisease,andaccumulationofpolyglutamine-containingaggregatesinHuntington’sdisease.

Antigen:FibrilspreparedfromhumanAβ42peptide.

HostSpecies:Rabbit

AntibodyClass:Polyclonal

Thisantibodyrecognizesepitopescommontomanyhumanamyloidfibrilsandfibrillaroligomersbutnotprefibrillaroligomersornativelyfoldedproteins.Predictedtorecognizemouseandratbasedonsequencehomology.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

Immunoblotting:useat1:1,000dilution.

Dotblot:useat1:1,000dilution.

DotblotanalysisofAβ42andpolyQ36prefibrillaroligomersandfibrils.Aβ42andpolyQfibrilsstainwith

#57005serum,whileAβ42andpolyQprefibrillaroligomersreactwith#57006(A11).

Immunohistochemistry:useat1:1,000dilution.

Labelingofamyloidfibrilswasobservedinthehippocampus(A),subiculum(B)andfrontalcortex(C)inAlzheimerdisease.Ahighermagnificationphotographillustratesthatamyloidfibrildepositsweredenseandconsistedoffinefibrillarmaterial(D).

ELISA:useat1:1,000dilutionwithamyloidfibril-containingsamplesonthesolidphase.Thesearerecommendedconcentrations.

Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisproductisstableforatleast1yearat

-20oC.Freezeinmultiplealiquotstoavoidrepeatedfreeze-thawcycles.