CatalogNo.:57006(A11)

Size:100ulpurifiedrabbitimmunoglobulin(0.5mg/ml)inphosphate-bufferedsaline,pH7.0,0.09%sodiumazideand50%glycerol.

Amyloidmonomericproteinscanoligomerizeintodestructiveamyloidfibrils.Amyloidogenicconformationsofnon-diseaserelatedproteinscanbecreatedbypartialproteinmisfoldingordenaturation.Manydegenerativediseasesareknowntoberelatedtotheaccumulationofmisfoldedproteinsasamyloidfibers.Theseincludetheamyloid-βpeptideplaquesandtauneurofibrillarytanglesinsenileplaquesofAlzheimer’ssymptomology,thedepositionofα-synucleinintheLewybodiesofParkinson’sdisease,andaccumulationofpolyglutamine-containingaggregatesinHuntington’sdisease.

Antigen:Syntheticmolecularmimicofsolubleoligomers.

HostSpecies:Rabbit

AntibodyClass:Polyclonal

Thisantibodyrecognizesapeptidebackboneepitopethatiscommontoalltypesofamyloidoligomersbutisnotfoundinnativeproteins,amyloidogenicmonomers,ormatureamyloidfibrils.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

Immunoblotting:useat0.5-1ug/ml

Aβ42fibrils(F)andprefibrillaroligomers(O)wererunonSDSpolyacrylamidegels,transferredtonitrocelluloseandprobedwith#57006(A11).

Dotblot:useat0.5-1ug/ml

DotblotanalysisofAβ42andpolyQ36prefibrillaroligomersandfibrils.Aβ42andpolyQfibrilsstainwith

#57005serum(OC),whileAβ42andpolyQprefibrillaroligomersreactwith#57006(A11).

ELISA:useat1-10ug/mlwithamyloidoligomer-containingsamplesonthesolidphase.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisproductisstableforatleast1yearat

-20oC.Freezeinmultiplealiquotstoavoidrepeatedfreeze-thawcycles.