
Ankyrin-BMonoclonalAntibody
PurifiedbyProteinGaffinitychromatography.
Ankyrinsareafamilyofadaptorproteinsthatmediatetheattachmentofintegralmembraneproteinstothespectrin-actinbasedmembraneskeleton.Ankyrinshavebindingsitesforthebetasubunitofspectrinandatleast12familiesofintegralmembraneproteins.Thislinkageisrequiredtomaintaintheintegrityoftheplasmamembranesandtoanchorspecificionchannels,ionexchangers,andiontransportersintheplasmamembrane.Ankyrin-Bproteinswereidentifiedinbrainandmuscle.
Ankyrin-BandAnkyrin-GproteinsarerequiredforthepolarizeddistributionofmanymembraneproteinsincludingtheNa+/K+ATPase,thevoltagegatedNa+channel,andtheNa+/Ca2+exchanger.
Thisantibodyrecognizeshuman,mouse,andratAnkyrin-B.Itdoesnotcross-reactwithAnkyrin-G.
Immunoblotting:useat1ug/ml.Predictedmolecularweightis>200kDa.
Immunoblotonmouseandratbrainlysateswith
#56538at1ug/ml.
PositiveControl:ratbrainlysate.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.