productdatasheet

CatalogNo.:2253

Size:100ugIgGin200ulPBS,pH7.4,purifiedbyimmunoaffinitychroma-tography.

Accumulationoftheamyloid-βpeptide(Aβ)inthecerebralcortexisacriticaleventinthepathogenesisofAlzheimer'sdisease.

Aβpeptideisgeneratedasaresultofproteolyticcleavageoftheβ-amyloidproteinprecursor(APP)atβ-andγ-sitesbytwoproteases.APPisfirstcleavedbyβ-secretasetoproduceasolublederivativeoftheproteinandamembrane-anchored99-aminoacidcarboxy-terminalfragment(C99).TheC99fragmentservesassubstratefor

γ-secretasetogeneratethe4kDamyloid-βpeptidewhichisfoundinbrainsofpatientswithAlzheimer’sdisease.β-secretasewasrecentlyidentifiedanddesignatedbeta-siteAPPcleavingenzyme(BACE)andaspartylprotease2(Asp2).BACE/Asp2isatransmembraneasparticproteaseandco-localizeswithAPP.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.

Antigen:Peptidecorrespondingtoaa485-

501ofhumanBACE.Thissequencediffersfromthoseofmouseandratbyoneaminoacid.

HostSpecies:Rabbit

StABIlizers:None

Preservatives:0.02%sodiumazide.

ThisantibodyrecognizeshumanandmouseBACE(approx.70kD).

Immunoblotting:useat1:500-1:1,000dilution.

Positivecontrol:Humanbraintissuelysate.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.

QEDBioscience,Inc.10919TechnologyPlace,SuiteC

SanDiego,CA92127

TollFree800.929.2114

Phone858.675.2405

Fax858.592.1509

info@qedbio.com

Visitourwebsiteforadditionalproductinformationandtoorderonline.

qedbio.com