Anti-BassoonPolyclonalAntibody
CatalogNo.:56495
Size:100uginPBS,pH7.4(1mg/ml).PurifiedbyProteinGaffinitychromatography.
Bassoon,a420kDaprotein,islocalizedatpresynapticnerveterminalsandappearstoplayaroleinthestructuralandfunctionalorganizationofthesynapticvesiclecycle.Bassoonispredictedtocontaintwodouble-zincfingers,threecoiled-coilregions,andtwopolyglutaminedomains.TheC-terminalregionsofthepolyglutaminedomainsareofinterestsinceabnormalamplificationofthisregioninsomehumanproteins,suchasHuntingtin,cancauselate-onsetneuro-degeneration.
Antigen:Fusionproteincorrespondingtoaa786-1041(N-terminal)ofhumanBassoon(accessionno.NM_003458.3).HostSpecies:Rabbit
AntibodyClass:Polyclonal
Thisantibodyrecognizeshuman,mouse,andratBassoon.Multipleisoformsaredetected.
APPLICATIONSImmunoblotting:useat1ug/ml.
Immunoblotonratandmousebrainlysates.Immunohistochemistry:useat1-10ug/ml.Immunofluorescence:useat1-10ug/ml.
Bassoon,BTX,andoverlay.Neuromuscularjunctionwholemusclesamplesfromadultmousewith#56495diluted1:400andAlexa488-conjugatedsecondaryantibody.
Positivecontrol:RatbraintissuelysateThesearerecommendedconcentrations;endusershoulddetermineoptimalconcentrationsfortheirapplications.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notintendedfordiagnosticortherapeuticapplications.
