
BrevicanMonoclonalAntibody
Brevicanisthemostabundantchondroitinsulfateproteoglycanintheextracellularmatrixoftheadultbrain.Itisamemberofthelecticanfamilyofaggregatingextracellularmatrixproteoglycansthatbearchondroitinsulfate(CS)chains.ItishighlyexpressedinthecentralnervoussystemandisthoughttostABIlizesynapsesandinhibitneuralplasticity.
Brevicanissecretedfromastrocytesandneuronsasa145kDacoreproteinthatbearsuptothreecovalently-linkedCSchains.Itisalsoissecretedasa145kDacoreproteinwithoutCSchains.Whencleavedbyextracellularglutamylendopeptidases,a55kDaN-terminalfragmentisformedthatcontainstheuniqueC-terminalepitopeEAMESE.
ThisantibodyrecognizesmouseandratBrevican.
Immunoblotting:useat1-2ug/ml.Abandof~140kDaisdetected.Smallercleavageproductsarealsodetected.
Positivecontrol:Ratbrainlysate.
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.