
CalnexinMonoclonalAntibody
Calnexinisa~90kDaintegralproteinoftheendoplasmicreticulum;itisalsoreferredtoasIP90,p88andp90.Itconsistsofa50kDaN-terminalcalcium-bindingluminaldomain,asingletransmembranehelix,and
ashortacidiccytoplasmictail.Itspositivelychargedcytosolicresiduesensureitsretentionintheendoplasmicreticulum.Calnexintogetherwithcalreticulinplayakeyroleinglycoproteinfoldingbyinteracting
withfoldingintermediatesviatheirmonoglycosylatedglycans.CalnexinhasalsobeenshowntoassociatewiththemajorhistocompatABIlitycomplexclassIheavychains,partialcomplexesoftheTcellreceptor,andBcellsurfaceimmunoglobulin.
Thisantibodyreactswithhumanand
Dictyosteliumdiscoideumcalnexin.
Immunoblotting:useat1-10ug/ml.
Immunohistochemistry:useat1-10ug/ml.
Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.
Reconstituteindistilledwater.
Thisproductisstableforatleastone(1)yearat-20oCto-70oC.Reconstitutedproductshouldbestoredinappropriatealiquotstoavoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.