Calnexinisa~90kDaintegralproteinoftheendoplasmicreticulum;itisalsoreferredtoasIP90,p88andp90.Itconsistsofa50kDaN-terminalcalcium-bindingluminaldomain,asingletransmembranehelix,and

ashortacidiccytoplasmictail.Itspositivelychargedcytosolicresiduesensureitsretentionintheendoplasmicreticulum.Calnexintogetherwithcalreticulinplayakeyroleinglycoproteinfoldingbyinteracting

withfoldingintermediatesviatheirmonoglycosylatedglycans.CalnexinhasalsobeenshowntoassociatewiththemajorhistocompatABIlitycomplexclassIheavychains,partialcomplexesoftheTcellreceptor,andBcellsurfaceimmunoglobulin.

Thisantibodyreactswithhumanand

Dictyosteliumdiscoideumcalnexin.

Immunoblotting:useat1-10ug/ml.

Immunohistochemistry:useat1-10ug/ml.

Thesearerecommendedconcentrations.Endusershoulddetermineoptimalconcentrationsfortheirapplications.

Reconstituteindistilledwater.

Thisproductisstableforatleastone(1)yearat-20oCto-70oC.Reconstitutedproductshouldbestoredinappropriatealiquotstoavoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.