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CaMKII,amemberofthecalcium/calmodulin-activatedproteinkinasfamily,functionsinneuralsynapaticstimulationandT-cellreceptorsignaling.CaMKIIisexpressedinmanytissues,butitisspecificallyfoundintheneuronsoftheforebrain,anditsmRNAisfoundwithinthedendritesandthesomaoftheneuron.
CaMKIIinneuronsconsistsoftwosubunitsof52(alphagenes)and60kDa(betagenes).CaMKIIhascatalyticandregulatorydomainsaswellasanATP-bindingdomainandaconsensusphosphorylationsite.
Bindingofcalcium/calmodulintoitsregulatorydomainreleasesitsauto-inhibitoryeffectandactivatesthekinase.Thiskinaseactivationresultsinautophosphorylationatthreonine286.
AutophosphorylationconfersenhancedaffinityofCaMKIIforNMDAreceptorsinpostsynapticdensities.
Thisantibodyrecognizesthephosphorylatedandnon-phosphorylatedformsofmouse,rat,andbovineCaMKII.
Immunoblotting:useat0.1-1ug/ml.Abandof~50-60kDaisdetected.
Immunohistochemistry:useat10ug/ml.
RIA:useat3-5ug/ml.
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:Ratbraintissueextract.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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