ORDERINGINFORMATION

Catalognos.:56370-50(50ug)56370-200(200ug)

Format:ProteinA-purifiedantibodyinPBS,50%glycerol,0.09%sodiumazide.

BACKGROUND

Calmodulin-dependentproteinkinaseII(CaMKII)isaserine/threonine-specificproteinkinasethatisregulatedbytheCa2+/calmodulincomplex.CaMKIIisinvolvedinmanysignalingcascadesandisthoughttobeanimportantmediatoroflearningandmemory.CaMKIIisalsonecessaryforCa2+homeostasisandreuptakeincardiomyocytes,chloridetransportinepithelia,positiveT-cellselection,andCD8T-cellactivation.CaMKIIhas28differentisoformsthatderivefromthealpha,beta,gamma,anddeltagenes.MisregulationofCaMKIIislinkedtoAlzheimer’sdisease,Angelmansyndrome,andheartarrhythmia.

SPECIFICATIONSUMMARY

Antigen:Syntheticpeptidecorrespondingtoaa7-20(N-terminus)ofratCaMKIIdeltaisoformconjugatedtoKLH.

Accessionno.:P15791

GeneID:25246

HostSpecies:Rabbit

AntibodyClass:Polyclonal

Specificity:Thisantibodyrecognizesalpha,delta,andgammaisoformsofhuman,mouse,rat,bovine,hamster,guineapig,chicken,andrabbitCaMKII.

APPLICATIONS

Immunoblotting:Useat1-5ug/ml.Abandof~50-60kDaisdetected.

Immunohistochemistry:useat1-10ug/ml.

DetectionofCaMKIIinhumanlung.

DetectionofCaMKIIinmousebrainextract(Lane2),ratbrainextract(Lane3),HeLacelllysate(Lane4),Hs-67celllysate(Lane5).

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplications.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisantibodyisstableforatleastone(1)yearat-20oC.Storeinappropriatealiquotstoavoidmultiplefreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.