productdatasheet
CD74isanon-polymorphictypeIIintegralmembraneprotein.IthasashortN-terminalcytoplasmictailof28aminoacidsfollowedbyasingle24-aatransmembraneregionandanapproximately150-aalumenaldomain.CD74functionsmainlyasanMHCclassIIchaperonewhichpromotesERexitofMHCclassIImoleculesbydirectingthemtoendocyticcompartments,preventingpeptidebindingintheER,andcontributingtopeptide“editing”intheMHCclassIIcompartment.CD74hasrecentlybeenshowntohavearoleasanaccessorysignalingmoleculebecauseofitshigh-affinitybindingtothepro-inflammatorycytokinemacrophagemigrationinhibitoryfactor(MIF).
28)conjugatedtoKLH.HostSpecies:MouseAntibodyClass:IgG1
Preservatives:0.09%sodiumazideOtheradditives:50%glycerolSPECIFICITYThisantibodyrecognizeshumanCD74.
Immunoblotting:useat1ug/ml.Adoubletbandof~33-35kDaisdetected.
Immunoprecipitation:useat12ug/ml.Immunocytochemistry:workingconcentrationissampledependent.Flowcytometry:workingconcentrationissampledependent.
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:PALAcelllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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