Copper-TransportingATPase1MonoclonalAntibody
CatalogNo.:56516(cloneS60-4)Size:100uginPBS(1mg/ml),pH7.4,50%glycerol,0.09%sodiumazide.
PurifiedbyProteinGaffinitychromatography.
APPLICATIONSImmunoblotting:useat1-2ug/ml.Abandof~180kDaisdetected.
BACKGROUND
ThecoppereffluxtransportersATP7AandATP7Bsequesterintracellularcopperintothevesicularsecretorypathwayforexportfromcells.ATP7A(alsoknownas
Copper-TransportingATPase1)functions
asatransmembranecopper-translocatingP-typeATPaseandplaysavitalroleinsystemiccopperabsorptioninthegutandcopperreabsorptioninthekidney.
AlthoughATP7Aisnotdetectableinmostnormaltissues,itisexpressedinmanycommontumortypes.IncreasedexpressionofATP7Arenderstumorcellsresistanttocisplatinandcarboplatin.
Antigen:Syntheticpeptidecorrespond-ingtoaa42-61(cytoplasmicC-terminus)ofhumanCopper-TransportingATPase1(accessionno.NP_000043.3).
HostSpecies:Mouse
AntibodyClass:IgG2b
Thisantibodyrecognizeshuman,mouse,andratCopper-TransportingATPase1.
Immunoblotonratbrainmembranes.
Immunohistochemistry:useat1-5ug/ml.Thesearerecommendedconcentrations.
Endusershoulddetermineoptimalconcentrationsfortheirapplications.Positivecontrol:ratbrainmembranes.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisproductisstableforatleast1yearat
-20oC.Freezeinmultiplealiquotstoavoidrepeatedfreeze-thawcycles.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
