CatalogNo.:56517(cloneS62-29)Size:100uginPBS(1mg/ml),pH7.4,50%glycerol,0.09%sodiumazide.

PurifiedbyProteinGaffinitychromatography.

ThecoppereffluxtransportersATP7AandATP7Bsequesterintracellularcopperintothevesicularsecretorypathwayforexportfromcells.ATP7b(alsoknownas

Copper-TransportingATPase2)transportscopperinandoutofcellsusingATP.Thereare3knownisoformsoftheATP7bgene:Aisfoundintheliver,kidneyandbrain,theshorterformBisfoundinbrain,andthethirdisoform,knownasWND/140KDAisfoundinmitochondria.

MutationsintheATP7bgenecancauseWilson’sdisease,aninheriteddisordercausingcopperpoisoninginthebrainandliver.

Antigen:Syntheticpeptidecorrespond-ingtoaa3-21(cytoplasmicN-terminus)ofhumanCopper-TransportingATPase2.(accessionno.NP_000044.2).

HostSpecies:Mouse

AntibodyClass:IgG1

Thisantibodyrecognizeshuman,mouse,andratCopper-TransportingATPase2.

Immunoblotting:useat1-2ug/ml.Abandof~160kDaisdetected.

Immunoblotonratbrainmembranes.

Immunohistochemistry:useat1-5ug/ml.Thesearerecommendedconcentrations.

Endusershoulddetermineoptimalconcentrationsfortheirapplications.Positivecontrol:ratbrainmembranes.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Thisproductisstableforatleast1yearat

-20oC.Freezeinmultiplealiquotstoavoidrepeatedfreeze-thawcycles.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.