productdatasheet

CatalogNo.:56101(4H9)and56102(5D5).

Size:100ugpurifiedantibodyinPBS,pH7.4.

Antigen:Purifiedhuman/ratcorticotropin-releasingfactor(CRF,Sigma).

HostSpecies:Mouse

AntibodyClass:IgG1

Purificationmethod:Threesequentialprecipitationswith40%ammoniumsulfate.Purifiedantibodiesyield1bandinnativeSDS-PAGE.

Preservatives:None.Availableonrequest.

TheseantibodiesreactspecificallywithCRF.Thereisnocross-reactivitywithbovineserumalbumin,insulin,ovalbumin,orepidermalgrowthfactor.ThesetwoantibodiesrecognizedifferentepitopesonCRF.Dissociationconstants=10-9Mfor56101and1.5x10-8Mfor56102.

TheseantibodiesmaybeusedinimmunoassaystodetectandquantitateCRF.Otherapplicationsareunderinvestigation.

DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.

Theseantibodiesarestableforatleastone(1)yearat-20oCto-70oC.Storeproductinappropriatealiquotstoavoidmultiplefreeze-thawcycles.

KravchenkoandFuralev.1994.

Hybridoma13:59-64;Furalevetal.

1994.BiochemBiophysResCom204:

823-834.

Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedure

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