productdatasheet
CatalogNo.:56462(cloneS151-3)Size:100uginPBS,pH7.4;50%glycerol,0.09%sodiumazide.PurifiedbyProteinGaffinitychromatography.
GABAisthemajorinhibitoryneuro-transmitterinthevertebratebrain.GABAreceptorsconsistoffivesubunitsthatformachlorideionchannel.Subunitfamiliesα,β,γ,andδhavebeenstudiedextensively;πandεhavebeenidentifiedrecently.
StudiesofrecombinantGABAreceptorshaveshownthatindividualsubunitsandtheirsubtypesconferdifferentsensitivitiestoGABAreceptormodulators.SubunitsubtypesaredifferentiallyexpressedthroughoutdevelopmentandindifferentCNSregions,reducingthetotalnumberofpossIBLeisoformsthatcanbeformedindifferentbrainregionsandinindividualcells.
SPECIFICATIONSUMMARYAntigen:Syntheticpeptidecorrespondingtoaa15-34ofratDeltaGABA-Areceptor(accessionno.P18506).HostSpecies:MouseAntibodyClass:IgG2aThisantibodyrecognizesmouseandratDeltaGABA-Areceptor.
APPLICATIONSImmunoblotting:useat1-10ug/ml.Abandof~55kDaisdetected.
RatcerebellarlysateImmunohistochemistryandImmunocytochemistry:useat0.1-1ug/ml
Immunofluorescence:useat1-10ug/ml
Rathippocampalneuronsurface(S)andtotal(T)proteinimmunofluorescentstaining.
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:Ratbrain.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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