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Theextracellularsignal-regulatedkinases1and2(ERK1/2)aremembersoftheMitogenActivatedProteinKinase(MAPK)familyofproteinsfoundinalleukaryotes.The44kDaERK1andthe42kDaERK2arehighlyhomologousandbothfunctioninthesameproteinkinasecascade,sotheyarereferredtocollectivelyasERK1/2.Theyarebothfoundinthecytosolandmitochondria.ERK1and2areactivatedbyMEK1orMEK2bydualphosphorylationofathreonineandtyrosineresidueintheactivationloop.Onceactivated,thesekinasesphosphorylateavarietyofsubstratesincludingcytoskeletalproteins,translationregulators,transcriptionfactors,andtheRskfamilyofproteinkinases.ERK1/2activationisbelievedtoconferasurvivaladvantagetocellsbutmayalsocontributetocelldeathundercertainconditions.
Thisantibodyrecognizeshuman,mouse,rat,bovine,chicken,sheep,Drosophila,andXenopusERK1/2.
Immunoblotting:useat1:5,000dilution.Bandsof~44kDa(ERK1)and~42kDa(ERK2)aredetected.
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
Positivecontrol:HeLacelllysate.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
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