ORDERINGINFORMATION

Catalogno.:56584(cloneS202-7)

Format:100ug(1mg/ml)ProteinG-purifiedantibodyinPBS,pH7.4,0.1%sodiumazide,50%glycerol.

BACKGROUND

TheproteinencodedbytheFIG4genebelongstotheSACdomain-containingproteingenefamily.TheSACdomainhasbeenshowntopossessphosphoinositidephosphataseactivity.Theyeasthomolog,Sac1p,isinvolvedintheregulationofvariousphosphoinositides,andaffectsdiversecellularfunctionssuchasactinCytoskeletonorganization,Golgifunction,andmaintenanceofvacuolemorphology.Membrane-boundphosphoinositidesfunctionassignalingmoleculesandplayakeyroleinvesicletraffickingineukaryoticcells.DiseasesassociatedwithFIG4includePolymicrogyria,BilateralTemporooccipitalandCharcot-Marie-ToothDisease,Type4J.

SPECIFICATIONSUMMARY

Antigen:Fusionproteincorrespondingtoaa688-907(C-terminus)ofmouseFIG4.Thissequenceis95%identicalinratand91%identicalinhuman.

Accessionnos:NP_598760.1,Q91WF7

GeneID:103199

HostSpecies:Mouse

AntibodyClass:IgG1

Specificity:Thisantibodyrecognizeshuman,mouse,andratFIG4.

APPLICATIONS

Immunoblotting:useat1-5ug/ml.Abandof

~100kDaisdetected.

Immunofluorescence:useat10ug/ml.

DetectionofFIG4inmousebrainlysatewith

#56584at5ug/ml.

Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.

DetectionofFIG4inneuroblastomacelllineSK-N-BEwith#56584at10ug/ml:DAPI(blue)nuclearstain,TexasRedFactinstain,ATTO488(green)FIG4stain.

DILUTIONINSTRUCTIONS

DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.

STORAGEANDSTABILITY

Thisproductisstableforatleastone(1)yearat-20oC.

Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.