ORDERINGINFORMATION
Catalogno.:56575(cloneS206B-9)
Format:100ug(1mg/ml)ProteinG-purifiedantibodyinPBS,pH7.4,0.1%sodiumazide,50%glycerol.
BACKGROUND
Glialfibrillaryacidicprotein(GFAP)isanintermediatefilamentproteinthatisexpressedbynumerouscelltypesofthecentralnervoussystem.ItisinvolvedinmanyimportantCNSprocesses,includingcellcommunicationandthefunctioningofthebloodbrainbarrier.Itiscloselyrelatedtoitsnon-epithelialfamilymembers,vimentin,desmin,andperipherin,whichareallinvolvedinthestructureandfunctionofthecell’sCytoskeleton.GFAPisthoughttohelptomaintainastrocytestrengthandshape.TherearemultipledisordersassociatedwithimproperGFAPregulation.GFAPlevelsareusedasaMarkerofneurologicdamageinadultswhosufferstrokesandtraumaticbraininjuries.
SPECIFICATIONSUMMARY
Antigen:Syntheticpeptidecorrespondingtoaa411-422(KTVEMRDGEVIK)ofhumanGFAP.Thissequenceis100%identicalinmouseandrat.
Accessionnos:NP_001124491.1,P14136
GeneID:2670
HostSpecies:Mouse
AntibodyClass:IgG1
Specificity:Thisantibodyrecognizeshuman,mouse,andratGFAP.
APPLICATIONS
Immunoblotting:useat1-5ug/ml.Abandof
~50kDaisdetected.
Immunofluorescence:useat10ug/ml.
DetectionofGFAPinratbrainlysatewith#56575at4ug/ml.
DetectionofGFAPinneuroblastomacelllineSK-N-BEwith#56575at10ug/ml:DAPI(blue)nuclearstain,TexasRedFactinstain,ATTO488(green)GFAPstain.
Thesearerecommendedconcentrations.Endusersshoulddetermineoptimalconcentrationsfortheirapplication.
DILUTIONINSTRUCTIONS
DiluteinPBSormediumthatisidenticaltothatusedintheassaysystem.
STORAGEANDSTABILITY
Thisproductisstableforatleastone(1)yearat-20oC.
Forinvitroinvestigationaluseonly.Notintendedfortherapeuticordiagnosticprocedures.
