
GluN2A/NR2AMonoclonalAntibody
N-methyl-D-aspartatereceptorsareheteromericproteincomplexes,andthreefamiliesofNMDARsubunitshavebeenidentified:NR1,NR2andNR3.ThesubunitcompositiondeterminesthepharmacologicalandphysiologicalpropertiesoftheNMDARs.NR2AisgraduallyincreasedinadultCNSbutisabsentinembryonicCNS.Electro-physiologicaldatasuggestthatNR2A-containingNMDARstendtobelocalizedsynaptically,whereasNR2B-containingreceptorstendtobelocalizedperi-synapticallyorextrasynaptically.Inthespinalcord,thepredominantsubunitsofNMDARsincludeNR2AatsynapticsitesandNR2Batextrasynapticsites.
Thisantibodyrecognizeshuman,mouse,andratGluN2A/NR2A.Itdoesnotcross-reactwithNR2B.
APPLICATIONSImmunoblotting:useat1ug/ml.Abandof
~170kDaisdetected.
Positivecontrol:Ratbrainlysate.
Thesearerecommendedconcentrations.Usershoulddetermineoptimalconcentrationsfortheirapplication.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidrepeatedfreezingandthawing.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.