productdatasheet
CatalogNo.:2117
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychroma-tography.
NuclearfactorkappaB(NF-κB)isaubiquitoustranscriptionfactorandkeymediatorofgeneexpressionduringimmuneandinflammatoryresponses.NF-κBactivatesnumerousgenesinresponsetoextracellularstimuli,suchasIL-1,TNFα,andLPS.NF-κBisassociatedwithIκBincytoplasm,whichinhibitsNF-κBactivity.IκBkinase(IKK),whichphosphorylatesIκBandmediatesIκBdegradationandNF-κBactivation,wasrecentlyidentified.IKKisaserineproteinkinase,andtheIKKcomplexcontainsalphaandbetasubunits(IKKαandIKKβ).IKKαandIKKβinteractwitheachother,andbothareessentialforNF-Bactivation.IKKαspecificallyphosphorylatesIκB-αandisexpressedinavarietyofhumantissues.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
SPECIFICATIONSUMMARYAntigen:Peptidecorresponding
toaa658-674ofhumanIKKαwhichdiffersfromthecorrespondingmurinesequencebyoneaminoacid.
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizeshumanIKKα(85kD).Nocross-reactivitywithIKKβorIKKγ.
Immunoblotting:useat1:500-1:1,000dilution.
Positivecontrol:WholecelllysatefromHeLaorJurkatcells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone
(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
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