productdatasheet
CatalogNo.:2329
Size:100ugIgGinPBS,pH7.4,purifiedbyimmunoaffinitychroma-tography.
NuclearfactorkappaB(NF-κB)isaubiquitoustranscriptionfactorandkeymediatorofgeneexpressionduringimmuneandinflammatoryresponses.NF-κBactivatesnumerousgenesinresponsetoextracellularstimuli,suchasIL-1,TNFα,andLPS.NF-κBisassociatedwithIκBincytoplasm,whichinhibitsNF-κBactivity.
IκBisphos-phorylatedbytheIκBkinase(IKK)complexwhichcontainsIKKα,IKKβ,andIKKγ.AnovelmoleculeintheIKKcomplex,designatedIKKε,isrequiredfortheactivationofNF-κBbyPMAandTcellreceptorsbutnotTNFαandIL-1.IKKεisexpressedinavarietyoftissuesandisinducIBLebyTNFα,IL-1,andLPS.
Forinvitroinvestigationaluseonly.Notforuseintherapeuticordiagnosticprocedures.
SPECIFICATIONSUMMARYAntigen:Peptidecorrespondingtoaa705-716ofhumanIKKε.
HostSpecies:Rabbit
StABIlizers:None
Preservatives:0.02%sodiumazide.
ThisantibodyrecognizeshumanIKKε(80kD).Nocross-reactivitywithIKKα,IKKβorIKKγ.
Immunoblotting:useat1:500-1:1,000dilution.
Positivecontrol:WholecelllysatefromJurkatcells.
DiluteinPBSormediumwhichisidenticaltothatusedintheassaysystem.
Thisantibodyisstableforatleastone(1)yearat-20oC.Avoidmultiplefreeze-thawcycles.
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